F7250
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F7250
Sigma
Fluorescein isothiocyanate isomer I
suitable for protein labeling, ≥90% (HPLC), powder
| Synonym: | FITC, Fluorescein 5-isothiocyanate |
| CAS Number: | 3326-32-7 |
| Linear Formula: | C21H11NO5S |
| Molecular Weight: | 389.38 |
| Beilstein Registry Number: | 1407295 |
| EC Number: | 222-042-0 |
| MDL number: | MFCD00005063 |
| PubChem Substance ID: | 24894933 |
Description
| Application | Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC) |
| Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. FITC is yellow-orange in color with an absorption maximum at 495 nm. Upon excitation it emits a yellow-green color with an emission maximum at 525 nm. |
Properties
| assay | ≥90% (HPLC) |
| form | powder |
| fluorescence | λex 492 nm; λem 518 nm (green) |
| mp | >360 °C(lit.) |
| suitability | suitable for protein labeling |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| WGK Germany | 3 |
References
| reference | , E. Harlow and D. Lane, ed. Antibodies: A Laboratory Manual Cold Spring Harbor, NY , (1988), 353-355 |
| Miller, L., Protein modification of actin at lysine-61. Eur. J. Biochem. 174, 23, (1988) | |
| Der-Balian, G.P., FITC labelling of Fab'. Anal. Biochem. 173, 59, (1988) | |
| Wilderspin, A.F., Green, N.M., Modification of thiol groups. Anal. Biochem. 132, 449, (1983) | |
| Muramoto, K., Microsequencing of proteins and peptides (HPLC). Anal. Biochem. 141, 446, (1984) | |
| Aldrich MSDS 1, 948:C / Corp MSDS 1 (1), 1688:D / FT-IR 1 (2), 469:C / FT-IR 2 (3), 4153:C / FT-NMR 1 (2), 1560:B / IR-Spectra (2), 995:C / IR-Spectra (3), 1142:D / NMR-Reference 2 (2), 440:C / RegBook 1 (2), 2157:I / Sigma FT-IR 1 (2), 148:B / Stains and Dyes Ref, 377 / Structure Index 1, 445:C:5 |
